Which method is used to measure total bilirubin?

Which method is used to measure total bilirubin?

• A. Wiener method
• B. Jendrassik-Grof method
• C. Modified Van den Berg method
• D. Direct spectrophotometry

Answer: (B)

The Jendrassik-Grof method is a widely accepted and standardized technique for measuring total bilirubin levels in serum or plasma. This method relies on the reaction of bilirubin with diazotized sulfanilic acid, resulting in a colored compound that can be quantified spectrophotometrically.

This approach is effective because it allows for the separation of total bilirubin into its conjugated and unconjugated fractions, thus providing an accurate total measure. The Jendrassik-Grof method is noted for its sensitivity and specificity, making it ideal for clinical laboratory settings, particularly in diagnosing and managing conditions like jaundice and liver disease.

In contrast, other methods such as the Wiener method and the Modified Van den Berg method may not offer the same level of accuracy and reliability for total bilirubin measurement. Direct spectrophotometry could measure bilirubin but lacks the specificity and robustness of the Jendrassik-Grof method. Hence, the Jendrassik-Grof method stands out as the preferred technique for total bilirubin assessment in clinical chemistry.